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. 2020 Mar 10;21(5):1894. doi: 10.3390/ijms21051894

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Conditions that affected the stability of Med15. (A) Expression levels of PTR2, PUT4, and YDJ1 extracted from RNAseq data from Supplemental Table S1. (B) Plasmids containing Med15^YJM789-Myc and Med15^S288c-Myc were transformed into single mutants of med15. The put4, ptr2, and ydj1 in the BY4741 background were grown and serial dilutions of yeast on YPD were grown for 2 days at 30 °C and then photographed. (C) Serial dilution of yeast knockouts of ydj1 yeast expressing YJM789 or S288c alleles of Med15-Myc on YPD. (D) Western blot of YJM789 or S288c alleles of Med15-Myc immunoprecipitated from BY4741 or the ydj1 mutant, which were grown in YPD.