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. 2020 Mar 10;21(5):1894. doi: 10.3390/ijms21051894

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Impact of Snf1 and Reg1 on yeast expressing different Med15 alleles. (A) Serial dilutions of BY4741 with different alleles of Med15 that were untagged or C-terminally tagged with 13xMyc grown on YPD or 400 ppm of MCHM and photographed after three days of growth. (B) Serial dilution of BY4741 with single and double mutants containing med15Δ, snf1Δ, and reg1Δ knockouts grown on YPD or 350 ppm of MCHM. Plates were photographed after two days of growth. (C) Serial dilutions of BY4741 snf1Δ mutants expressing different alleles of Med15 with and without the 13xMyc tag grown on YPD or 350 ppm of MCHM. Plates were photographed after three days of growth. (D) Western blot of YJM789 or S288c alleles of Med15-Myc immunoprecipitated from BY4741 grown in YPD with SNF1 or REG1 deleted.