Figure 7.

Quantitative growth assays of BY4741 med15 carrying different alleles of Med15 in snf1 and reg1 mutants with different drugs. At the time, when there was the maximum growth difference, the OD₆₀₀ of yeast carrying Med15^S288c was subtracted from the OD₆₀₀ of yeast carrying Med15^YJM789. Values above the y-axis indicate increased growth of the yeast with Med15^S882c and values below the y-axis indicate that the yeast with Med15^YJM789 grew better than yeast with the other allele. The following chemicals were added: hydrogen peroxide (H₂O₂), 4-nitroquinoline 1-oxide (4NQO), 4-methylcyclohexanol (MCHM), copper sulfate (CuSO₄), rapamycin (Rapa), glyphosate (CR41), hydroxyurea (HU), beta-mercaptoethanol (βME), calcofluor white (CALC), caffeine (CAFF), dithiothreitol (DTT), and hygromycin (HYG). (A) Growth of BY4741 med15 yeast carrying different alleles of Med15-Myc. (B) Growth of BY4741 med15 yeast carrying different alleles of Med15 (untagged, blue) or Med15-Myc (tagged, orange). (C) Growth of BY4741 med15 and snf1 yeast carrying different alleles of Med15 (untagged, light blue) or Med15-13xMyc (tagged, light orange). The respective values of wildtype yeast from panel (B) are shown as a thin red line with the standard deviation range shown as thin red lines. (D) Growth of BY4741 med15 and reg1 yeast carrying different alleles of Med15 (untagged, cyan) or Med15-Myc (tagged, brown). The respective values of wildtype yeast from panel (B) are shown as a thin red line with the standard deviation range shown as thin red lines.