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Supplementary Data, Figure 1. Endonuclease assays. Reactions mixtures (10 μl) contained 50 mM Tris (pH 7.5), 50 mM NaCl, 1 mM DTT, 5 μM SpikeTRS, 25 ng of the indicated protein. Reactions were stopped by the addition of 90% formamide, and electrophoresed through a 6 M urea, 20% acrylamide high resolution gel. The positions of the SpikeTRS and the 4 nt cleavage product are indicated on the right.
