Fig. 3.
Glycosylation of the expressed H6HA1-His.
The molecular mass of the expressed H6HA1-His decreased when the glycosylation sites were mutated. (A) Mutants in which one glycosylation site (N167A) or all four sites (M4) were deleted expressed H6HA1-His proteins with smaller molecular mass. (B) The expressed protein was treated with glycosidase (PNGase-F) to remove the glycan; both molecular mass and band intensity decreased. (C) A schematic showing four possible glycosylation sites on H6HA1.