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. 2018 Jun 9;459:81–89. doi: 10.1016/j.jim.2018.06.001

Fig. 3.

Fig. 3

Glycosylation of the expressed H6HA1-His.

The molecular mass of the expressed H6HA1-His decreased when the glycosylation sites were mutated. (A) Mutants in which one glycosylation site (N167A) or all four sites (M4) were deleted expressed H6HA1-His proteins with smaller molecular mass. (B) The expressed protein was treated with glycosidase (PNGase-F) to remove the glycan; both molecular mass and band intensity decreased. (C) A schematic showing four possible glycosylation sites on H6HA1.