Fig. 6.

Gel filtration analysis of the H6HA1-His protein from hemolymph.

(A) After ammonium sulfate fractionation (Supplementary Fig. S4), the proteins were separated by gel filtration, which revealed one large protein peak and several minor peaks (blue curve). Three standards (a, b, and c) were used as molecular weight markers, and they were applied under the same conditions (orange curve). (B) Selected fractions were analyzed using SDS-PAGE and then Western blotting to reveal H6HA1-His proteins. CBR, Coomassie Blue R staining. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)