Fig. 2.

Desmethylicaritin inhibits adipogenesis-induced accumulation of lipids in 3T3-L1 preadipocytes at day 8 after adipogenic induction. (A) Representative morphological changes of 3T3-L1 adipocyte differentiation using Oil Red O staining. (B) The amount of accumulated lipid measured in terms of the absorbance of Oil Red O extracted from stained cells. (C) Proliferation of adipogenic cells at 2 days after treatment by adipogenesis differentiation medium. *P<0.05, **P<0.01, ***P<0.001 compared with the positive control (ctl+) that is induced by adipogenic induction but without desmethylicaritin treatment. NS: no significance.