Fig. 2.

ICP0 can activate AP-1 responsive reporter gene in different cells. (a) Schematic representation of AP-1-Luc luciferase reporter gene used in this study. Note that the AP-1 sequence of this construct is an authentic one that is slightly different from the AP-1 site identified in Fig. 1. ICP0 was co-transfected into cells along with this reporter gene and internal control for 48 h. Shown in panel (b) was the mean fold induction of the AP-1-Luc expression by gradient amount of ICP0 in neuroblastoma SH-SY5Y cells. The same luciferase assay experiments were carried out in (c) Hela cells and (d) 293T cells. The gradient expression of ICP0 and the loading control were shown in panels.