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. 2011 Aug 12;373(1):79–88. doi: 10.1016/j.jim.2011.08.005

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Copyright © 2011 Elsevier B.V. All rights reserved.

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Fig. 3 — Screening of Humanyx library against PA and Etox. (A) Polyclonal phage ELISA showing specific enrichment with subsequent pans for both PA and Etox antigens (B) number of total screened, positive (OD > 0.5) and unique clones (as determined by sequencing of variable regions) obtained. For the Etox library only clones OD > 1.0 were selected for further screening due to the large number of positives. (C) Monoclonal ELISA of selected anti-PA and Etox clones showing 100% retention of high affinity and specificity after conversion to IgG from Fab.