Fig. 2.

Heat maps of SELDI-TOF MS profiles of single sheep sera at week 0 and weeks 3 and 9 p.i. with Fasciola hepatica. Heat maps of all 100 statistically significant clusters from the pH 9, pH 7, pH 5 and organic wash fractions are shown. Biomarkers found to be statistically significant in two independent analyses were determined by comparison of individual spectra from weeks 0, 3 and 9 p.i. using Ciphergen Express software. At each time-point the data are presented in rank order of increasing worm burden (sheep 12, 24, 16, 13, 22, 25, 20 and 30). Data are shown in the range 1.5–10 kDa (a), 7–30 kDa (b) and 30–150 kDa (c). Heat maps are derived from analysis of randomly spotted samples. Peaks are clustered using dendograms (not shown) to reveal patterns of up-regulation (red) and down-regulation (green).