Table 2.

Summary of the 26 validated biomarkers identified in two separate fractionations of sheep sera ordered by M/z from both the first analysis (non-randomized spotting) and second analysis (randomized spotting) of individual serum fractions

Fraction	M/z	1st run		2nd run
		P-value	ROC	P-value	ROC
Week 3
pH 9	3005.7∗	0.039	0.797	0.019	0.750
pH 9	5684.9∗	0.013	0.984	0.013	0.938
Organic	16,946.5∗	0.013	0.984	0.019	0.938
Organic	17,138.4∗	0.013	0.984	0.019	0.891
Organic	18,011.0∗	0.013	0.984	0.019	0.891
Organic	36,144.5∗	0.019	0.797	0.013	0.984
Week 9
Organic	1832.4	0.013	0.984	0.013	0.703
pH 9	2025.2	0.019	0.063	0.013	0.016
pH 9	2317.7	0.013	0.938	0.013	0.844
Organic	2773.0	0.013	0.938	0.019	0.938
pH 9	2892.9	0.013	0.891	0.013	0.891
pH 9	3005.9∗	0.013	0.891	0.013	0.891
Organic	3342.9	0.013	0.063	0.013	0.016
pH 9	3341.2	0.013	0.063	0.013	0.063
pH 9	4629.4	0.013	0.938	0.013	0.938
pH 7	4629.3	0.027	0.891	0.027	0.844
pH 9	4716.2	0.013	0.891	0.013	0.891
pH 9	5685.6∗	0.013	0.984	0.013	0.984
Organic	8281.5	0.013	0.984	0.013	0.984
Organic	12,718.6	0.013	0.938	0.013	0.891
pH 9	13,784.7	0.013	0.844	0.019	0.891
Organic	16,948.7∗	0.013	0.984	0.019	0.844
Organic	17,144.2∗	0.013	0.984	0.027	0.844
Organic	18,012.8∗	0.013	0.984	0.013	0.984
Organic	36,150.3∗	0.013	0.984	0.013	0.984
pH 9	44,273.9	0.039	0.844	0.019	0.891
pH 9	45,295.6	0.039	0.844	0.013	0.938
Organic	55,033.6	0.019	0.938	0.027	0.844
pH 9	77,179.1	0.019	0.891	0.027	0.844
Organic	82,495.9	0.019	0.063	0.013	0.016
pH 9	88,020.3	0.027	0.891	0.039	0.844
pH 9	89,741.3	0.027	0.859	0.039	0.844

P-values < 0.05 are considered statistically significant. Biomarkers with ROC values above 0.5 are termed up-regulated biomarkers and biomarkers with ROC values below 0.5 are termed down-regulated biomarkers. Week 3 and week 9 biomarkers are shown. Peaks marked with ^∗ indicate that the peak is present at both weeks 3 and 9 as their M/z are within the error of the instrument (0.1%).