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. 2005 Mar 29;579(11):2404–2410. doi: 10.1016/j.febslet.2005.02.080

Figure 2.

figure image

Inhibition of SARS‐CoV N protein expression by siRNA expression cassettes. The 293T cells were co‐transfected with N‐EGFP expression plasmid and various PCR products. The SARS‐CoV N protein expression was measured. (A) N protein expression was detected by Western blot at 48 h post‐transfection. The upper panel shows N protein expression (lane 1 shows N expression in cells transfected with pN‐EGFP alone, other lanes show that in cells co‐transfected with pN‐EGFP and various PCR products, U6P denotes PCR product of mouse U6 promoter only). The lower panel shows β‐actin expression (as an endogenous control). (B) At 48 h post‐transfection, the total RNA was isolated and subjected to reverse transcription and real‐time PCR analysis. The mRNA levels of cells transfected with plasmid pN‐EGFP along with various PCR products were quantitated relative to that in the cells transfected with plasmid pN‐EGFP alone.