Table Table 1.
The siRNA target sequences and the reverse primers for preparation of siRNA expression cassettes
| siRNA | Sequence | Reverse primer sequence a |
|---|---|---|
| N186 | 5′‐GGAGGAACTTAGATTCCCT‐3′ | 3′ primer 1:5′‐TCTCTTGAAAGGGAATCTAAGTTCCTCCGGCCCAAACAAGGC‐3′ |
| 3′ primer 2:5′‐AAAAAAGGAGGAACTTAGATTCCCTTCTCTTGAAAGG‐3′ | ||
| N388 | 5′‐GGCATCGTATGGGTTGCAACT‐3′ | 3′ primer 1:5′‐TCTCTTGAAAGTTGCAACCATACGATGCGGCCCAAACA AGGC‐3′ |
| 3′ primer 2:5′‐AAAAAAGGCATCGTATGGGTTGCAACTTCTCTTGAAAGTTG‐3′ | ||
| N587 | 5′‐GAAATTCAACTCCTGGCAG‐3′ | 3′ primer 1:5′‐TCTCTTGAACTGCCAGGAGTTGAATTTCGGCCCAAACAAGGC‐3′ |
| 3′ primer 2:5′‐AAAAAAGAAATTCAACTCCTGGCAGTCTCTTGAACTG‐3′ | ||
| N811 | 5′‐GTCACTCAAGCATTTGGGAGA‐3′ | 3′ primer 1:5′‐TCTCTTGAATCTCCCAAATGCTTGAGTGACGGCCCAAACAAGGC‐3′ |
| 3′ primer 2:5′‐AAAAAAGTCACTCAAGCATTTGGGAGATCTCTTGAATCT‐3′ | ||
| S1184 | 5′‐GACAAATAGCGCCAGGACA‐3′ | 3′ primer 1:5′‐TCTCTTGAATGTCCTGGCGCTATTTGTCGGCCCAAACAAGGC‐3′ |
| 3′ primer 2:5′‐AAAAAAGACAAATAGCGCCAGGACATCTCTTGAATGT‐3′ | ||
| muN388 | 5′‐GGCATGCTATGGGTTGCAACT‐3′ | 3′ primer 1:5′‐TCTCTTGAAAGTTGCAACCATAGCATGCGGCCCAAACA AGGC‐3′ |
| 3′ primer 2:5′‐AAAAAAGGCATGCTATGGGTTGCAACTTCTCTTGAAAGTTG‐3′ |
a
3′ primer 1 denotes the reverse primer for the first round PCR, 3′ primer 2 denotes the reverse primer for the second round PCR.