Fig. 1.
Intranasal and intramuscular immunization with recombinant adenovirus-based vaccine expressing OVA induces distinct OVA-specific memory CD4 T cells. 2 × 106 LN cells from naïve DO11.10 mice were transferred i.v. to naïve BALB/c mice. Recipient mice were immunized with 5 × 108 pfu AdOVA i.n. or i.m. 24 h post-adoptive transfer (5 mice per group). Four weeks post immunization, single cell suspensions were prepared from the alveolar space, lung parenchyma and spleen and restimulated with OVA323-339 peptide for 72 h. (a) The concentrations of IFN-γ, IL-13, IL-17, IL-6, TNF-α and IL-10 in culture supernatants were determined by ELISA. The differences between groups were analyzed using two-way ANOVA test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). (b/c) The frequencies (b) and total number (c) of DO11.10 CD4 T cells in single cell suspensions were determined by flow cytometry.