Fig. 3.

The route of immunization has potent effects on the quality of CD4 T cells. DO11.10:BALB/c mice were immunized with 5 × 10⁸ pfu or 1 × 10⁹ pfu of AdOVA i.n. or i.m. for 5 days (3 mice per group). (a) The percentage and the number of KJ1-26⁺ cells in the MedLN and IngLN of naïve or immunized DO11.10:BALB/c mice were determined by flow cytometry. (b) The representative dot plots show the frequency of DO11.10 cells. (c) The percentage and the number of KJ1-26⁺ cells in the spleen of naïve or immunized DO11.10:BALB/c mice. (d) Splenocytes were stimulated with 5 μg/mL OVA peptide for 6, 12, 24 and 48 h. IFN-γ concentration was determined by ELISA. The data are expressed as the mean ± SEM (n = 3) and the differences between groups were analyzed using two-way ANOVA test. ^$$p < 0.01 between indicated groups, ^∗p < 0.05, ^∗∗p < 0.01 compared to naïve mice.