Fig. 5.

AdOVA immunization induces respiratory macrophage migration and accumulation in the MedLN and lung parenchyma. CFSE-labeled BALB/c mice were immunized with AdOVA i.n or PBS for 40 h. Single cell suspensions were prepared from the MedLN and lung and stained for flow cytometry. (a/c) Representative dot plots to show the gating strategy and the characteristics of CSFE-positive cells in the DLN (a) or lung (c). (b) The number of CFSE-positive migrating cells in the MedLNs of AdOVA immunized and PBS-treated mice. (d) The number of respiratory macrophage populations and DCs in the lung of AdOVA immunized and PBS-treated mice. The results are representative of two independent experiments with 4 mice per group. The differences between groups were analyzed using two-way ANOVA test (^∗∗p < 0.01, ^∗∗∗p < 0.001).