Table 2.
Bacterial culture and multiplex PCR for viruses and Mycoplasma
| Pigs with CBP (n = 148) |
Control animals (n = 60) |
P value∗ | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Acute |
Subacute |
Chronic |
|||||||||||||||
| ASBP (n = 10) |
SSBP (n = 16) |
SMBP (n = 6) |
SNBP (n = 2) |
CSBP (n = 52) |
CMBP (n = 37) |
CNBP (n = 25) |
|||||||||||
| n | % | n | % | n | % | n | % | n | % | n | % | n | % | n | % | ||
| Bacterial culture | |||||||||||||||||
| P. multocida† | |||||||||||||||||
| + | 0 | – | 2 | 13 | 1 | 17 | 0 | – | 4 | 8 | 4 | 11 | 6‡ | 24 | 0 | – | 0.004 |
| ++ | 2 | 20 | 5‡ | 31 | 3‡ | 50 | 1 | 50 | 5 | 10 | 2 | 5 | 3 | 12 | 1 | 2 | 0.004 |
| +++ | 7 | 70 | 8 | 50 | 1 | 17 | 1 | 50 | 31 | 60 | 18 | 49 | 10 | 40 | 1 | 2 | <0.001 |
| S. suis | 0§ | – | 4 | 25 | 2 | 33 | 0 | – | 7 | 13 | 8 | 22 | 6 | 24 | 6 | 10 | NS |
| S. aureus | 0 | – | 0 | – | 0 | – | 1 | 50 | 0 | – | 0 | – | 0 | – | 0 | – | NS |
| [H.] parasuis | 0 | – | 0 | – | 0 | – | 0 | – | 2 | 4 | 2 | 5 | 1 | 4 | 0 | – | NS |
| A. pleuropneumoniae | 0 | – | 0 | – | 0 | – | 0 | – | 1 | 2 | 0 | – | 0 | – | 0 | – | NS |
| Mixed culture | 2 | 20 | 0 | – | 0 | – | 0 | – | 0 | – | 4 | 11 | 0 | – | 27 | 45 | <0.001 |
| Sterile | 0 | – | 0 | – | 0 | – | 0 | – | 5 | 10 | 3 | 8 | 4 | 16 | 25 | 42 | <0.001 |
| Multiplex PCR | |||||||||||||||||
| PCV2 | 10 | 100 | 14 | 88 | 6 | 100 | 2 | 100 | 50 | 96 | 37 | 100 | 24 | 96 | 56 | 93 | NS |
| PRRSV | |||||||||||||||||
| EU type | 1 | 10 | 3 | 19 | 0 | – | 0 | – | 2 | 4 | 1 | 3 | 1 | 4 | 0 | – | NS |
| US type | 0 | – | 0 | – | 0 | – | 0 | – | 1 | 2 | 0 | – | 0 | – | 0 | – | NS |
| PCMV | 3 | 30 | 3 | 19 | 1 | 17 | 0 | – | 15 | 29 | 7 | 19 | 6 | 24 | 26 | 43 | NS |
| SIV-A | 0 | – | 0 | – | 0 | – | 0 | – | 0 | – | 2 | 5 | 1 | 4 | 1 | 2 | NS |
| M. hyopneumoniae | 10 | 100 | 15 | 94 | 6 | 100 | 1 | 50 | 51 | 98 | 35 | 95 | 24 | 96 | 21 | 37 | <0.001 |
| M. hyorhinis | 9 | 90 | 13 | 81 | 6 | 100 | 2 | 100 | 41 | 79 | 30 | 81 | 17 | 68 | 22 | 35 | <0.001 |
Numbers and percentage of positive animals are listed for the 148 pigs with CBP in relation to diagnosis.
ASBP, acute suppurative bronchopneumonia (BP); SSBP, subacute suppurative BP; SMBP, subacute mixed BP; SNBP, subacute non-suppurative BP; CSBP, chronic suppurative BP; CNBP, chronic non-suppurative BP; CMBP, chronic mixed BP. PRRSV (EU type, European type; US type, North American type); SIV-A, swine influenza virus type A; NS, not significant; PCV2, porcine circovirus type 2; PCMV, porcine cytomegalovirus.
P values for differences between cases and controls were calculated by either the Chi-square or Fischer's exact test as appropriate.
Growth of P. multocida was graded as weak growth (+), dense growth (++) or marked growth (+++).
The frequency of each pathogen detected in the diagnosis group was tested against the remaining cases of CBP by the Chi-square or Fischer's exact test. Significant associations are marked by ‘‡’.