Fig. 1.

(a) Characteristic NOE restraints derived from a ¹⁵N-edited 3D NOESY–HSQC experiment. (b) Ribbon representation of the energy minimized average NMR model of MAYV MD. (c) Backbone superposition of MDs from MAYV (gray; PDB 5IQ5), CHIKV (orange; PDB 3GPG), SINV (blue; PDB 4GUA),and VEEV (beige; PDB 3GQE) showing also the degree of sequence conservation in four loops. (d) Representation of the electrostatic surface potential of MAYV MD (red, − 4; blue, + 4; calculated with the PyMol APBS extension). (e) S² order parameters of the backbone of MAYV MD. Residues with values close to one have a decreased mobility on the ps/ns timescale. (f) Mapping of residues with slowly exchanging backbone amide protons in the core region of MAYV MD (dark red). The corresponding ¹H,¹⁵N HSQC spectra at time points 11 and 1125 min of the H/D exchange experiment are shown below.