Fig. 4.

Effect of CH on host Hsc70 mRNA. HepG2 cells were transiently transfected with the wild-type or lamivudine-resistant HBV genome and then treated with cepharanthine hydrochloride (CH) at concentrations of 0 (1), 0.01 (2), 0.1 (3), 1 (4), 10 (5) or 100 (6) μM for 6 days. At the end of treatment, the total RNA was extracted, and heat stress cognate 70 (Hsc70) mRNA was determined by RT-PCR (A and B). The levels of Hsc70 mRNA were quantified and normalized relative to the internal reference GAPDH mRNA (C and D). The experiments were performed in triplicate, and a representative set of data is presented. **P < 0.01, *P < 0.05 compared with the no drug control group.