Table 1.
Sequences of Primers and Predicted Sizes for Target Genes
Chemokine/Primer | Predicted Size (Base Pairs) |
---|---|
CXCL10 | |
Forward, 5′-TGAATCAAACTGCGATTCTG-3′ | 275 |
Reverse, 5′-TTTCCTTGCTAACTGCTTTCAG-3′ | |
G3PDH | |
Forward, 5′-ACCACAGTCCATGCCATCAC-3′ | 450 |
Reverse, 5′-TCCACCACCCTGTTGCTGTA-3′ |
The amplification reaction was performed for 30 cycles, with denaturation at 94°C for 30 s, annealing at 58°C for 30 s, and extension at 72°C for 30 s.