Table 1.
Sequences of Primers and Predicted Sizes for Target Genes
| Chemokine/Primer | Predicted Size (Base Pairs) |
|---|---|
| CXCL10 | |
| Forward, 5′-TGAATCAAACTGCGATTCTG-3′ | 275 |
| Reverse, 5′-TTTCCTTGCTAACTGCTTTCAG-3′ | |
| G3PDH | |
| Forward, 5′-ACCACAGTCCATGCCATCAC-3′ | 450 |
| Reverse, 5′-TCCACCACCCTGTTGCTGTA-3′ | |
The amplification reaction was performed for 30 cycles, with denaturation at 94°C for 30 s, annealing at 58°C for 30 s, and extension at 72°C for 30 s.