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. 2015 Dec 16;141(1):121–130. doi: 10.1378/chest.10-3297

Table 1.

Sequences of Primers and Predicted Sizes for Target Genes

Chemokine/Primer Predicted Size (Base Pairs)
CXCL10
 Forward, 5′-TGAATCAAACTGCGATTCTG-3′ 275
 Reverse, 5′-TTTCCTTGCTAACTGCTTTCAG-3′
G3PDH
 Forward, 5′-ACCACAGTCCATGCCATCAC-3′ 450
 Reverse, 5′-TCCACCACCCTGTTGCTGTA-3′

The amplification reaction was performed for 30 cycles, with denaturation at 94°C for 30 s, annealing at 58°C for 30 s, and extension at 72°C for 30 s.