Fig. 1.

Description of the LAMP-Tube scanner. The tube scanner assembled with temperature settings to amplify DNA isothermally and spectral tools to distinguish amplified product using fluorescence is shown (A). The tube scanner can hold 8, 200 μL PCR tubes and is assembled with an LCD screen through which positive or negative results can be seen. If the tube scanner is connected to a computer with the suitable software, the results are obtained in real-time as shown in (B). The fluorescence units are shown on the Y-axis and time to amplification on the X-axis. Amplification curves are observed (curved lines, Plasmodium genus specific primers – blue lane, Plasmodium falciparum species specific primers – magenta color) in case of positive samples. No amplification curves (flat line, orange color) indicated a negative sample. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)