Fig. 2.

Amplification of the two most human-infecting Plasmodium species using the LAMP-Tube scanner method. Plasmodium species-specific primers were used to amplify the 18s ribosomal RNA gene in P. falciparum and P. vivax parasites. Amplification curves (positive) were observed for the two species (100 parasitemia/μL) within 30 min (vertical dotted line), and with the low parasitemia (40 & 10 p/μl), amplification curves were seen in 40 min (vertical dotted line). No amplification was seen having the negative control or in the no template control (NTC). As a positive control (PC), purified Plasmodium falciparum DNA (3D7) was amplified in 18 min.