Fig. 3.

ARID2 ectopic expression inhibits HCC cell proliferation and invasion. ARID2 expression plasmid or pcDNA3.1 empty vector was transfected in Hep3B and HepG2 cells. At 48 h after transfection, the cells were harvested and analyzed. (A) ARID2 and IFITM1 protein level was detected by Western blot; GAPDH was used as a loading control. (B, C) Cell proliferation was analyzed by flow cytometry (B) and colony formation assays(C). (D) Transwell assay was performed to analyze the invasion ability of HCC cells. The experiments were independently repeated thrice. *P<0.05.