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Trans‐cleavage of V‐ATPase G1 TRX fusion protein by SARS‐CoV 3CLpro protease. The SARS‐CoV 3CLpro protease was incubated with the V‐ATPase G1 TRX fusion protein (lanes 6–9) or TRX protein (lanes 2–5) in 100 mM Tris–HCl, (pH 9.0) at 37 °C, and the proteolytic processing was analyzed using Western blotting with anti‐His tag antibody. Lane 1, protein markers.
