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. 2005 Jul 27;128(3):708–716. doi: 10.1053/j.gastro.2004.12.007

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Copyright © 2005 American Gastroenterological Association. Published by Elsevier Inc. All rights reserved.

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Effects of shRNA on HBV RNA expression and DNA replication. (A) Northern blot analysis of HBV transcripts from HuH-7 cells cotransfected with pHBV48 and shRNA-expressing plasmids as indicated. PC represents positive control and is a 200 pg, 3.2-kb HBV DNA fragment. The same blot was hybridized with a glyceraldehydes phosphate dehydrogenase (GAPDH) DNA probe as an internal loading control. This blot represents the results of 4 similar experiments. (B) Southern blot analysis of HBV replicative intermediates in cytoplasm or virion-associated HBV DNA in culture supernatant of HuH-7 cells transfected with pHBV48 and shRNA-expressing plasmids. PC represents DNA samples from HuH-7 cells transfected with pHBV48 alone. This blot represents the results of 2 similar experiments.

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