Figure 6.

(A) Target sequences of pSuper/HBVS1 on HBV-CI-wt and HBV-CI-m. CI denotes a genotype C HBV from the patient I, and “m” and “wt” denote resistant mutant and wild type, respectively. The triplet codons for surface and polymerse genes are illustrated by lines located either above (S open reading frame) or below (polymerase open reading frame) the sequence. The mutated nucleotide on the mutant is underlined. The nucleotide change from T to C does not change the amino acid sequences of either surface or polymerase genes. Both GTT and GTC encode for valine in the S gene; TTG and CTG encode for leucine in the polymerase gene. (B) Sequence electropherogram produced by sequencing PCR products directly. HuH-7 cells were transfected with pHBV-CI-wt and pHBV-CI-m at a 9:1 ratio in the presence of pSuper or pSuper/HBVS1. Viral DNA extracted from virions released from transfected cells on day 2 and day 5 posttransfection were PCR amplified with primers HBV+2836 and HBV-695 then sequenced with HBV+56. *Indicates position of mutated sequence.