Fig. 5.

Workflow of the process for detecting genomic DNA using single molecule arrays (SiMoA). The target DNA, fragmented by the use of restriction enzymes, is mixed with biotinylated detection probes at an elevated temperature to form single-stranded DNA. The DNA-probe mixture is then incubated with magnetic beads decorated with DNA probes. Hybridized DNA complexes are then labelled with beta-galactosidase via biotin-streptavidin interaction. These beads are then loaded into arrays of femtoliter wells, and single DNA fragments are detected. Reproduced with permission from Ref. [145], http://dx.doi.org/10.1021/ac303426b.