Fig. 1.

C-terminal 2A* compromises the expression of ER targeted proteins. (a) Schematic representation of the non-conventional translation termination caused by FMDV peptide 2A, resulting in the release of the upstream protein terminating in Gly (G) and the translation of the downstream protein initiating with Pro (P). (b) Left panel: SV5-tagged secretory reporter constructs (pr1) fused to the C-terminal peptide 2A followed by a STOP-codon (2A*) or including a P-codon before the STOP-codon (2A-P*). Right panel: Western blot of cell culture supernatants and extracts of HEK293T cells transfected with the plasmid constructs shown. (c) Quantification of the data shown in panel b. Data presented as mean ± S.E.M. of n = 3 independent experiments. (d) Western blot of extracts from cells transfected with constructs encoding as a reporter the SV5-tagged membrane protein MHC-Iα fused to each of the two C-terminal 2A* or 2A-P* sequences. Data are representative of n = 3 independent experiments.