Fig. 2.

2A* causes ribosomal stalling at the STOP-codon. (a) Schematic representation of constructs containing the 11-aa-long roTag (left panel) and the corresponding Western blots (right panel, representative of three independent experiments) of supernatants and extracts of transfected cells. Black and red arrowheads indicate conventional and non-conventional translation termination products, respectively. (b) Scheme of constructs (left panel) and the corresponding Western blots (right panel, representative of three independent experiments) of extracts of transfected cells. Red arrowheads indicate non-conventional termination of pr1 in constructs A and B. (c) Autoradiography of anti-SV5 immunoprecipitates obtained from total extracts of cells transfected with the indicated constructs and labeled with [³⁵S]-methionine for 15 min. Quantification is indicated at the bottom of each lane. Representative of n = 2 independent experiments.