Fig. 3.

Stalling is independent of mRNA codon usage. (a–c) Western blots of cell extracts and/or supernatants, as indicated, derived from cells transfected with the control construct 2A-P* or variants of constructs 2A*, with different STOP-codons (a) and G-codons (b) and with degenerated codons for all amino acids of 2A sequence, shown also for 2A-P* (c). (d) Western blots of extracts and supernatants of cells transfected with constructs containing the Teschovirus-derived 2A sequence. (e) Schematic representation of the two new constructs (*2A-P* and *2A*, left panel) and the corresponding Western blots (right panel) of supernatants of transfected cells. Black arrowheads indicate translation termination. Data in all panels are representative of at least n = 3 independent experiments.