Fig. 4.

Mapping the minimal 2A amino acid sequence causing ribosomal stalling. (a) Scheme of constructs with 2A variants. (b–e) Western blots of supernatants of cells transfected with the indicated constructs. (f) Scheme of constructs (left panel) and Western blot (right panel) of extracts of cells transfected with the indicated variants. Black and red arrowheads indicate conventional and non-conventional termination, respectively. Data in all panels are representative of at least n = 2 independent experiments.