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. 2018 Oct 24;431(2):123–141. doi: 10.1016/j.jmb.2018.10.009

Fig. 8.

Fig. 8

The products of membrane-bound stalled ribosomes are bound by BiP/GRP78. (a) Scheme of the secretory version of BioID2 constructs (left panel) and StrAv Western blot of extracts of cells transfected with the indicated plasmids and labeled with biotin for 24 h. The rightmost panel shows a zoom of the 75-kDa region with lower exposure. Black arrow indicates the self-biotinylation of the BioID2 protein, while red arrows indicate bands that appear only in extracts of cells transfected with BioID2-2A*. Representative of n = 3 experiments. (b) Western blots with anti-roTag antibody and StrAv-HRP of extracts shown in panel a. (c) Western blots of extracts of cells mock-transfected or transfected with the indicated BioID2 constructs: upper panel, anti-BiP immunoblotting of proteins pulled-down with StrAv beads; middle and lower panels, StrAv-HRP or anti-BiP blots of proteins immunoprecipited (IP) with anti-BiP. Data are representative of n = 2 independent experiments.