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. 2020 Mar 25;15(3):e0230450. doi: 10.1371/journal.pone.0230450

Fig 3. Over-expressions of HNF-4α inhibit hepatocellular carcinoma cells proliferation through regulating miR-122.

Fig 3

(A) HNF-4α, ADAM17, EGFR, AKT, p-AKT, F-Caspase 3 and C-Caspase 3 protein expression in HepG2 cells transfected with HNF-4α plasmid for 24 h. (B) qRT-PCR analysis of miR-122 expression in HepG2 cells transfected with HNF-4α plasmid for 24 h. Relative quantification of western blot results HNF-4α (C), ADAM17 (D), EGFR (E), p-AKT/AKT (F), and cleaved caspase 3/caspase 3 (G) levels expressed relative to control. (H) MTT assay of HepG2 cells transfected with HNF-4α plasmid for 24 h in vitro. (*P < 0.05 vs. the control). CON: control; NC: negative control.