Fig 3. Chromatin remodeling activities and the recruitment of transcription machinery at INO1 promoter are independent of Snf2p acetylation.

Real-time PCR analysis of DNA immunoprecipitated through ChIP with an antibody against (A) Histone H3 (α-H3); (B) Histone H4 (α-H4); (C) Acetylated Histone H3 (α-acH3); (D) Acetylated Histone H4 (α-acH4); (E) RNA polymerase II (α-Pol II) at the INO1 promoter for WT cells, unAcSnf2p, and snf2Δ cells that were grown to mid-log phase (A₆₀₀ = 1.0) in SC and were subsequently washed and subjected to repressing (+ino; open bars) or inducing (-ino; light grey bars) conditions, respectively, for 2 hours prior to collection. All experiments were performed with three repeats and all PCR reactions were done in at least triplicate. The IP for the INO1 promoter is graphed as mean ± standard deviation normalized to input and mock.