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. 2020 Mar 13;16(3):e1008374. doi: 10.1371/journal.ppat.1008374

Fig 5. Infection with kanamycin propagated A. baumannii results in reduced cytokine and chemokine production in the lung and blood.

Fig 5

The indicated cytokines and chemokines were quantified from whole lung homogenates (a) and blood (b) of mice at four- and 12-hours following infection with WT propagated in lysogeny broth, Tn5A7 propagated in lysogeny broth containing kanamycin, or co-infected with an equal mixture of the two strains. (c) BMDMs were infected with the indicated bacterial strains at a multiplicity of infection of 15. Infected cells were incubated for 4 hours at which time the cell culture supernatant was removed and assayed. (d) Mice were treated with intraperitoneal injection of 50 μg of anti-IL-10 antibody or isotype control at the time of infection with WT mixed with killed WT or mixed with killed Tn5A7 propagated in medium containing kanamycin. Columns depict the mean and error bars show standard deviation of the mean. Means were compared with the mean of WT at that time using a one-way ANOVA adjusted for multiple comparisons. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not statistically significant.