Figure 2. The active inhibitor 1 binds to genotype 1b HCV NS5A whereas the inactive stereoisomer 2 does not.
Genotype 1b replicon cells were exposed to either 1 or 2 for approximately 18 h before the cells were lysed. A portion of the supernatant was set aside to serve as input control for immunoblot analysis (lanes 1 and 2) while the remainder was mixed with streptavidin–agarose beads and incubated for several hours. Bound proteins were detected by immunoblotting with primary antibodies directed to HCV NS5A. Lanes 1 and 3 depict the results of experiments with the active inhibitor compound 1 whereas lanes 2 and 4 depict the results with the inactive stereoisomer 2.