Figure 3. Neutrophils move to sites of sterile injury by intravascular crawling.

(a) Time-lapse images using spinning-disk confocal intravital microscopy show the response of neutrophils (green) to focal hepatic necrosis (red, propiodium iodide). Scale bar, 200 μm (ref. 37). (b) Images of neutrophils homing (green) to sterile injury (red, propiodium iodide) in the liver of untreated mice (left) or after treatment with the fMLP receptor inhibitor cyclosporine H (right). (c) Neutrophils within the liver vasculature can prioritize an fMLP gradient when they are subjected to competing gradients of CXCL2 and fMLP, thus allowing them to chemotax toward the site of focal necrosis.