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. 2005 Jan 23;11(2):160–166. doi: 10.1038/nm1179

Figure 3. Shedding activity of ACE on the cell surface.

Figure 3

(a) Expression of EGFP-GPI was examined by FACS analysis. Purple area, ACE (−); green line, ACE (+); yellow line, PI-PLC-treated; black dot, background. (b) Expression of EGFP-GPI after ACE or PI-PLC treatment examined by fluorescence microscopy. Note that the fluorescence of Golgi complex remained the same. Magnification, ×200. (c) ACE caused shedding of various endogenous GPI-anchored proteins. The amount of ACE used was equivalent to the endogenous ACE activity. Values are mean ± s.d., n = 3. ND, not determined.