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. 2005 Jan 23;11(2):160–166. doi: 10.1038/nm1179

Figure 5. Involvement of ACE GPIase activity in sperm-egg binding.

Figure 5

(a) Distribution of Tesp-5 (TESP5) and Ph-20 (PH-20) in the sperm of wild-type and Ace knockout mice detected by immunoblotting. The acrosin and fertilin-β are indicated for water-soluble and detergent-soluble fraction controls, respectively. +/+, wild-type; −/−, Ace knockout. DS, detergent-soluble fraction; WS, water-soluble fraction. (b) Binding of Ace-knockout sperm to the zona pellucida after various treatments. The amount of Ace used for treatment was equivalent to the endogenous ACE activity. (c) Number of sperm bound to the egg. Values are mean ± s.e.m. *P < 0.001, **P < 0.005, compared with buffer control; P < 0.3, comparison of wild-type ACE with ACE-E414D; P < 0.5, comparison of wild-type ACE with PI-PLC; P < 0.05, comparison of PI-PLC with PI-PLC + Inositol-P (Student's t-test).