Figure 7.

The levels of β-catenin and YAP decreased in the striola of P8 utricles upon treatment with EGF and the GSK3 inhibitor CHIR-99021. a, Low-magnification images illustrating that the intensities of β-catenin and YAP immunostaining decreased in the striola of P8 mouse utricles cultured in EC medium, which did not occur in control medium. b, High-magnification images centered on the line of the polarity reversal (yellow dashes) depicting the decrease in β-catenin and YAP immunostaining at the cellular level. c, Orthogonal view of P8 mouse utricle cultured in EC that illustrates decrease in β-catenin and YAP immunostaining intensities in the striola and presence of EdU⁺ striolar SCs. d, β-catenin and YAP S/L ratios decreased in P8 mouse utricles cultured in EC medium compared with utricles cultured in control medium (n = 4 for Control and EC). e, Low-magnification images of P8 mouse utricles cultured in EC medium for 7 d with EdU and nocodazole added for the final 24 h of culture. Insets, Cells that progressed from S-phase to mitosis (EdU⁺/PH3⁺ cells). f, EdU⁺/Sox2⁺/PH3⁺ cells were present only in utricles cultured in EC medium (n = 4 for Control and EC). Data are mean ± SD. ns, Not significant (p ≥ 0.05). ***p < 0.001.