Figure 3.

Combined imaging/electrophysiology during temperature-induced seizures. Local field potential recording indicates that the imaging correlates of behavioral seizures correspond to electrographic seizures. We performed ECoG (see Materials and Methods) combined with in vivo 2P imaging, with an intracortical recording electrode placed within the 3 mm craniotomy window. A, Example recording showing a period of rhythmic spike and sharp wave activity corresponding to an electro-clinical seizure. B, Simultaneous 2P imaging during the electrical recording shown above, before seizure onset (Bi), at electrographic seizure onset (Bii), onset of large-scale recruitment of neuronal activity within the imaging field (Biii), and after cessation of the electrographic event (Biv).