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. 2020 Mar 25;6(13):eaaz2598. doi: 10.1126/sciadv.aaz2598

Fig. 3. 3D reconstructions of biomimetic microvessels with ECs illustrating uniformity of cell coverage and intercellular junctional morphology.

Fig. 3

Reconstructions of confocal Z-stacks of biomimetic microvessels populated by HPMECs, featuring the following: (A) VE-cadherin at endothelial adherens junctions (antibody labeling, green), (B) F-actin (phalloidin, red), and (C) merged images that include nuclei [4′,6-diamidino-2-phenylindole(DAPI), gray scale]. Insets in (A) and (B) show the cross-sectional views in the corresponding color channel and demonstrate the amount of overlap due to the roll-up process. Scale bars, 100 μm. A green-magenta rendering of this figure is shown in fig. S6.