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. 2020 Mar 25;6(13):eaaz2129. doi: 10.1126/sciadv.aaz2129

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 2 — (A) Tamoxifen treatment regimen in 8-week-old Skp1^f/− Ddx4-Cre^ERT2 males. (B) Testis weight of adult Skp1^cKO males. (C to J) Histological analysis of control (C) and Skp1^cKO (D to J) adult testes. Stage XII (C and D) and stage XI (E and F) tubules are shown. Please note that early-stage (I to VII) tubules from control and Skp1^cKO testes at 2, 4, and 6 dpt contained round spermatids. Scale bar, 25 μm. Z, zygonema; D, diplonema; M, metaphase; ES, elongated spermatid; MNC, multinucleated giant cells. (K) Surface nuclear spread analysis of control and Skp1^cKO spermatocytes. The plot shows the percentage of each type of spermatocytes. Scale bar, 10 μm.