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. 2020 Mar 25;6(13):eaaz2129. doi: 10.1126/sciadv.aaz2129

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Fig. 4 — Surface nuclear spread analysis of WT and Skp1^cKO (6 dpt) spermatocytes was performed by immunostaining of SYCP3 and γH2AX (A), RPA2 (B), and MLH1 (C). The percentage of different types of diplotene spermatocytes is shown as graphs. The count of MLH1 foci in MLH1-positive pachytene and diplotene spermatocytes is shown in a dot plot (C). Scale bars, 10 μm.