CS-56 (mouse) |
1:500 |
Sigma (C8035) |
2H6 (mouse) |
1:500 |
Amsbio (370710-IEC) |
Iba1 (rabbit) |
1:500 |
Wako (019–19747) |
GFAP (rabbit) |
1:500 |
Dako (Z0334) |
GFAP (chicken) |
1:500 |
Abcam (ab4674) |
Phosphacan (mouse) |
1:50 |
Developmental Studies Hybridoma Bank (3F8) |
Neurocan (biotinylated sheep) |
1:100 |
R&D systems (BAF5800) |
Aggrecan (rabbit) |
1:200 |
Chemicon (AB1031) |
Brevican (sheep) |
1:10 |
R&D systems (AF4009) |
Versican (rabbit) |
1:100 |
Chemicon (AB1032) |
Secondary antibodies |
1:1,000 |
Thermo Fisher (O-6381) |
goat anti-rabbit, Oregon Green 488 |
1:1,000 |
Thermo Fisher (A-21070) |
goat anti-rabbit, Alexa Fluor 633 |
1:1,000 |
Thermo Fisher (A-11039) |
goat anti-chicken, Alexa Fluor 488 |
1:500 |
Abcam (ab98749) |
goat anti-mouse, Alexa Fluor 633 |
1:1000 |
Thermo Fisher (A-21050) |
streptavidin-488 |
1:200 |
Thermo Fisher (S11223) |
donkey anti-sheep, Alexa Fluor 488 |
1:2500 |
Thermo Fisher (A-11015) |