Table 2.
Primer sequences
| Gene name | Forward primer | Reverse primer | Accession # |
|---|---|---|---|
| Actb* | GCCCTGAGGCTCTTTTCCA | TAGTTTCATGGATGCCACAGGA | NM_007393.3 |
| Dgat2 | ACTCTGGAGGTTGGCACCAT | GGGTGTGGCTCAGGAGGAT | NM_026384.3 |
| Fasn | GGGTTCTAGCCAGCAGAGTC | TCAGCCACTTGAGTGTCCTC | NM_007988.3 |
| Foxo1 | CAATGGCTATGGTAGGATGG | TTTAAATGTAGCCTGCTCAC | NM_019739 |
| G6pc | GCCTCCTGTCGGATACAGAA | TGCACCGCAAGAGCATT | NM_008061.4 |
| Gapdh* | TGACGTGCCGCCTGGAGAAA | AGTGTAGCCCAAGATGCCCTTCAG | NM_008084.2 |
| Insr | GTGTTCGGAACCTGATGAC | GTGATACCAGAGCATAGGAG | NM_010568 |
| Pepck | AGCGGATATGGTGGGAAC | GGTCTCCACTCCTTGTTC | NM_011044.2 |
Actb, beta-actin; Dgat2, diglyceride acyltransferase; Fas, fatty acid synthase; Foxo1, forkhead box O1; G6pase, glucose 6-phosphatase; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Insr, insulin receptor; Pepck, phosphorenolpyruvate carboxykinase.
*
Denotes reference genes.