Table 2.
Voltage-dependence of activation and inactivation parameters recorded in the presence of 100 nM 4,9-ah-TTX and absence (control) via whole-cell patch clamp from HEK cells stably expressing human (h) Nav1.1, Nav1.3 or Nav1.6. Values represent the mean ± S.E.
| V1/2 of Activation (mV) | k (mV−1) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| control | N | 4,9-anhydro-TTX (100nM) | N | control | N | 4,9-anhydro-TTX (100nM) | N | ||
| hNav1.1 | − 17.36 ± 1.05 | 9 | −15.31 ± 0.83 | 7 | 5.501 ± 0.27 | 9 | 5.705 ± 0.14 | 7 | |
| hNav1.3 | − 16.88 ± 1.58 | 7 | −14.47 ± 1.25 | 7 | 5.261 ± 0.24 | 7 | 5.332 ± 0.28 | 7 | |
| hNav1.6 | − 17.97 ± 0.80 | 7 | −17.28 ± 0.99 | 7 | 4.857 ± 0.28 | 7 | 6.265 ± 0.41* | 7 | |
| V1/2 of Activation (mV) | k (mV−1) | ||||||||
| control | N | 4,9-anhydro-TTX (100nM) | N | control | N | 4,9-anhydro-TTX (100nM) | N | ||
| hNav1.1 | − 50.41 ± 2.37 | 9 | −45.14 ± 1.55 | 7 | − 5.04 ± 0.22 | 9 | − 4.83 ± 0.55 | 7 | |
| hNav1.3 | − 53.41 ± 2.22 | 7 | −55.97 ± 1.95 | 6 | − 5.85 ± 0.59 | 7 | − 9.16 ± 1.51 | 6 | |
| hNav1.6 | − 50.73 ± 1.54 | 7 | −55.83 ± 2.79 | 5 | − 4.63 ± 0.41 | 7 | − 8.61 ± 2.17 | 5 | |
*
p = 0.0158.