Table 1.

A schematic depiction of the number of animals used in each experimental group.

Experiment	Age of animals	Treatment and sacrificed time point		Number of animals	Note
Astrocyte separation and culture	Neonatal	Within 24 h of birth		42
HE, IHC, and IHF staining	Neonatal	Virus	4 dpi	4
		PBS		4
Cytokine and monoamine detection	Neonatal	Virus	4 dpi	11	Half brain for cytokine, and half for monoamine test
		Inactivated Virus		7
		PBS		13
Cytokine and monoamine detection	Adult	Virus	4 dpi	4
		PBS		6
Viral load	Neonatal	Virus	2 hpi	3
			24 hpi	4
			48 hpi	4
			72 hpi	4
			96 hpi	3
			120 hpi	3
	Adult		2 hpi	4
			24 hpi	4
			48 hpi	4
			72 hpi	3
			96 hpi	3
			120 hpi	3
Survival	Neonatal	PBS		11	Figure 4a
		FY-22		16
		IL-6		10
		FY-22 + IL-6		14
		PBS		10	Figure 4b
		FY-22		16
		AD		12
		FY-22 + AD		16
		PBS		7	Figure 4c
		FY-23		10
		FY-23 + IL-6Ab		9
		PBS		8	Figure 4d
		FY-23		11
		FY-23 + Phenoxybenzamine		10

dpi days post infection, hpi hours post infection, AD adrenaline