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. 2020 Mar 25;10:5435. doi: 10.1038/s41598-020-61073-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup for social preference in adult zebrafish. The focal fish was placed in the test tank, and a stimulus shoal was placed in one of the side tanks, while the opposite side tank contained no fish. Visual barriers were placed between tanks and removed after the acclimation period was complete. Zones were designated as Zone 1 (the zone closest to the stimulus shoal) through to Zone 5 (the zone furthest away from the shoal).