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. 2020 Mar 25;10:5428. doi: 10.1038/s41598-020-62112-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spectra of color phenotypes and the corresponding SERDS spectra. The top (a,b), middle (c,d), and bottom (e,f) rows correspond to green, yellow, and white color phenotypes, respectively. The left column (a,c,e) corresponds to raw measured spectra taken with two slightly different laser excitation wavelengths. The right column (b,d,f) shows the corresponding reconstructed Raman spectrum (top red curve in each frame) retrieved using the SERDS technique compared to spectra generated from a conventional Asymmetric Least Squares (AsLS) algorithm (bottom curves in each frame). For the baseline-corrected spectra, only those features in each spectrum that correspond to a Raman response are sensitive to slight changes in laser excitation frequency.